Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. But sometimes these parameters are not sufficient enough. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. Incubate overnight at RT. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Read & analyse. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. * For HTRF microplate recommendations, please visit . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. VICTOR® Nivo™ multimode plate reader. Its. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Its. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Seal the plate and incubate 3 hours at RT . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Filters or monochromators for emission wavelength. (donor) of compounds are measured on an HTRF-compatible reader. Plate cells Stimulate cells 3. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. -CERTIFIED READER IS NEEDED. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. 5 hours before a further 3 washing steps. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. For more information about HTRF. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Assay setup for a 384-well low-volume plate. Specialized filters allow for IR fluorescence. FOR HTRF CERTIFIED. For more. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. FOR HTRF CERTIFIED READER . The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. (665 nm and 620 nm) on a compatible HTRF ® reader. These plates can be read on all HTRF certified readers, thus making their implementation in. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . VICTOR® Nivo™ multimode plate reader 2 | For research use only. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . Highly compatible plates for HTRF. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Read & analyse. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. FOR HTRF CERTIFIED READER. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Read & analyse. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). The VICTOR Nivo is a HTRF certified microplate reader. Plate 25 μL of cells in. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. FOR HTRF CERTIFIED READER For more information about HTRF. HTRF Signal + 5 hours @ RT or . Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. Transcreener® ADP2 TR-FRET assay. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Make sure the setup for Tb Cryptate is used. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Revvity. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PROTOCOL AT A GLANCE. Make sure the setup for Eu3+ Cryptate is used. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. This protocol. FOR HTRF CERTIFIED READER For. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. This protocol enables the cells' viability and confluence to be monitored. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 55 µL anti-cAMP-Cryptate. The plate was covered and incubated for one hour at room temperature. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Preconfigured HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. It measures at any wavelength, with top and bottom reading for most standard technologies. Page 30 4. No washing steps are required. Incubate overnight at RT. In particular, these parameters should be entered as below. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Tb Cryptate is used. Catalog No. FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Read & analyse. These convert photons generated by fluorescence or. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Due to the chemical and photophysical. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Make sure the setup for Tb Cryptate is used. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Incubate overnight at RT. Plate 25 μL of cells in. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. 2. a one- plate assay protocol. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Make sure the setup for Tb Cryptate is used. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. These plates can be read on all HTRF certified readers, thus making their implementation in. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. Each well requires 10 μL of 4X lysis buffer /well. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. 5 µL. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. The assay was prepared in both white and black plates and incubated >18 hours. com HTRF Microplate Readers. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Alpha Technology: The VICTOR Nivo system. FOR HTRF CERTIFIED READER. Plate Reader Requirement. no. The HTRF ratio obtained represents the total binding. Incubate 4h at RT. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Certified for use with Alpha, LANCE ® or DELFIA ®. 5 μL of Binding buffer 2X. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. + 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. View Brochure. Read & analyse. Plate Type and Read Area should be selected based on the microplate used and wells to be read. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Eu Cryptate is used. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. $352. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For. + 4 µL HTRF ® pre-mixed antibodies . 5 nm filter (PerkinElmer 2100-5110) were used. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Partnerships for delivering turn-key solutions. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. We would like to show you a description here but the site won’t allow us. Dual monochromators for assay flexibility With. The Negative control is used to check the non-specific signal. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. It can also be further streamlinedFOR HTRF CERTIFIED READER. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. 4. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Other technologies such as imaging. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Endpoint mode experiments measure each well of the microplate only once. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. 68. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. HTRF is compatible with a large range of available microplate readers. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Revvity. Revvity. FOR HTRF CERTIFIED READER. Rating: 5. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information. Dual-Luciferase® (DLR™) gene assays. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. The donor and acceptor can be used to label a variety of biomolecules, for applications. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. The lysis buffer 4X is ready-to-use. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. In this case the Z´(pronounced Z prime) value comes to mind. For more. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . For more information about HTRF. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 5% and an increased Z’ value of 0. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Customer Transformation Hub. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. best fluorescence polarization plate reader on the market. Incubate 4h at RT. Read & analyse. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. It features a modular design that allows users to add or change detection cartridges according to their needs. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. 15 / Each of 1; Qty. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Read & analyse. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. This protocol gives the cells viability and confluence to be monitored. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. . At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Plate 25 μL of cells in. 1) . Incubate overnight at RT. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. For instance, when assay volumes are. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF plate reader. Figure 3. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Combined with our Kaleido™ workflow software, it. The VICTOR Nivo is a HTRF certified microplate reader. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Plate 25 μL of cells in. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. It features a modular design that allows users to add or change detection cartridges according to their needs. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. Microplate reader assays requiring infrared (IR) or. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Read & analyse. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. HTRF-specific. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. MATERIALS PROVIDED: KIT . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER For more information about HTRF. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. For more information about HTRF. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. HTRF certified . They can be used in a no-transfer protocol with suspension cells or with. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. * For HTRF microplate recommendations, please visit . 5 hours before 3 more washing steps. Revvity. FOR HTRF CERTIFIED READER For more. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. At Cisbio we provide microplates that help you discover better. Typical applications The HTRF cartridge provides optimal performance for key applications. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Fluorescence intensity – Fluorescence intensity is one of the most. These plates can be read on all HTRF certified readers, thus making their implementation in. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. FOR HTRF CERTIFIED. SV detection plate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. For more information about HTRF. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. FOR HTRF CERTIFIED READER For more. At that stage, the analyte is sandwiched between the 2 antibodies. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. For more. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The adapterResults summary for HTRF IP1 standard curve. CLARIOstar is equipped with a specific optical device which enables the measurement of.